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|Section2= |Section3= }} Dipicolinic acid (pyridine-2,6-dicarboxylic acid or PDC and DPA) is a chemical compound which composes 5% to 15% of the dry weight of bacterial spores.〔Sci-Tech Dictionary. ''McGraw-Hill Dictionary of Scientific and Technical Terms'', McGraw-Hill Companies, Inc.〕 It is implicated as responsible for the heat resistance of the endospore.〔〔Madigan, M., J Martinko, J. Parker (2003). ''Brock Biology of Microorganisms'', 10th edition. Pearson Education, Inc., ISBN 981-247-118-9.〕 However, mutants resistant to heat but lacking dipicolinic acid have been isolated, suggesting other mechanisms contributing to heat resistance are at work.〔Prescott, L. (1993). ''Microbiology'', Wm. C. Brown Publishers, ISBN 0-697-01372-3.〕 Dipicolinic acid forms a complex with calcium ions within the endospore core. This complex binds free water molecules, causing dehydration of the spore. As a result, the heat resistance of macromolecules within the core increases. The calcium-dipicolinic acid complex also functions to protect DNA from heat denaturation by inserting itself between the nucleobases, thereby increasing the stability of DNA.〔Madigan. M, Martinko. J, Bender. K, Buckley. D, Stahl. D, (2014), Brock Biology of Microorganisms, 14th Edition, p. 78, Pearson Education Inc., ISBN 978-0-321-89739-8.〕 Two genera of bacteria are known to produce endospores: the aerobic Bacillus and anaerobic Clostridium.〔Gladwin, M. (2008). ''Clinical Microbiology Made Ridiculously Simple'', MedMaster, Inc., ISBN 0-940780-81-X.〕 Adrian Ponce, a chemist at the Jet Propulsion Laboratory has developed a technique for the detection of germinable endospores by staining dipicolinic acid with terbium. When the area is illuminated under ultraviolet light, live endospores glow in the presence of the terbium. The technique was developed for the validation of Spacecraft cleanliness, but it promises applications for the assessment of sterility more generally. The technique has the advantage of much greater speed over culture-based methods of endospore detection. It is also used to prepare dipicolinato ligated lanthanide and transition metal complexes for ion chromatography.〔(2,6-Pyridinedicarboxylic acid ) at Sigma-Aldrich〕 ==References== 抄文引用元・出典: フリー百科事典『 ウィキペディア（Wikipedia）』 ■ウィキペディアで「Dipicolinic acid」の詳細全文を読む スポンサード リンク
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